Established
2006
Franchise Units
130
Minimum Investment
$134,645
Franchise Fee
$60,000
Total Investment Range
$221,095
Home Based
No
Description
Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion.
Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities.
Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.
Franchise Service Lines:
- Crime scene cleanup
- Suicide cleanup
- Blood spill cleanup
- Hoarding cleanup
- Virus and bacterial disinfection
- Decomposition and undiscovered death
- Emergency vehicle decontamination
- Odor removal
- Teargas cleanup
- Rodent droppings
- Feces and urine cleanup
- Homeless encampment cleanup
- Biorecovery mold remediation
- Medical waste
Key Differentiators:
- First to Franchise Category Leader
- Niche specialized services in demand
- Purpose-Driven Team and Owner Network
- Comprehensive Training, Support, and Coaching
- Backed by Five Star Franchising and Princeton Equity
Top 5 Selling Points:
- Fast Startup with Low Initial Investment
- Home-based and Low Overhead
- Multiple Service Lines = Multiple Revenue Streams
- No Experience Needed
- Robust Training and Support
Background
Established: 2006
First Unit Franchised: 2010
Franchised Units: 130+
Company-Owned Units: 0
Registration Status
States Registered In:
Filed or filing with all registration states except Hawaii
(Confirmation required from primary contact for latest status)
Availability
- Canada Franchises: No
- International Franchises: No
Support Training
Bio-One – Training, Support & Marketing Overview
Initial Training (B.O.T.S) + OSHA Safety Training
New franchise owners travel to corporate headquarters for comprehensive, hands-on training designed to fully prepare them for daily operations.
What You Learn During Initial Training
- How to price, estimate, sell, and market services effectively
- Detailed training on chemicals, tools, and equipment used in field operations
- Hands-on simulated exercises to practice responding to various biohazard jobs
- Opportunity to observe a live service job (based on availability)
OSHA Safety Standard Training
- Conducted quarterly
- Mandatory for every Owner or Designated Manager
- Ensures compliance with federal safety standards and best practices
Ongoing Support
Bio-One provides continuous, long-term support designed to help franchisees grow and operate efficiently.
Network & Operational Support
- Full support throughout the entire life of the franchise agreement
- Access to an experienced franchise network that collaborates like a family
- Train-the-trainer programs, webinars, town halls, regional meetings, and Home Services Summit
- Systemwide KPI sharing, private communication groups, and frequent internal updates
- Dedicated billing specialist who handles communication with insurance adjusters
Marketing & Communications Support
Bio-One equips franchise owners with a complete marketing foundation from day one.
Branding & Digital Marketing
- Onboarding support for:
- Website
- SEO
- PPC Advertising
- Google Business Profile
- Online Listings
- Facebook & Social Media
- National branding guidelines and cohesive marketing strategy
- National sponsorships, partnerships, and brand-focused events
Local Marketing Tools
- Local advertising resources
- Professional marketing materials
- Guidance on community outreach and local visibility
Ideal Candidate
Candidates should enjoy giving back to others and be interested in making a difference in their community. Local networking skills, organization, confidence, and strong work ethic are helpful.
Day In the Life
- Make a daily marketing plan - Decide which locations to market to, how much marketing material to bring, and what your plan of attack is for the day.
- Market - Based on which community you will be marketing to, practice your pitch accordingly. Bring along your little black book of marketing and your business and rack cards.
- Call your team - If you get a call at any time of day, it’s your job to make sure your technicians are ready to arrive on a job site quickly.
- Suit up for a job - When arriving for a job, you should have the proper PPE. We work with chemicals to clean and disinfect dangerous germs and pathogens. Safety is a requirement. ALWAYS.
- Clean a bio job - Each call for a bio job is unique. Make sure you bring the right chemicals and equipment and you’ll be set to get the home back to normal.
- Clean a hoarding job - A typical hoarding job can take a few days to complete. Clean, organize, and sanitize and you’ll be on your way.
- Send an invoice - Price your job reasonably and send your invoice out. Work with your client or their insurance to get paid.
Financial Detail
Franchise Fee: $60,000
Recommended Liquid Capital: $60,000+
Total Investment Range:
$134,645 – $221,095
Veteran & First Responder Incentives
- Veteran Discount: $9,000 off the Franchise Fee
-
First Responder Discount: $9,000 off the Franchise Fee
(Both discounts not applicable to commission-level models)
Royalty Structure (Scaling Model)
Greater of:
- $750/month (Months 1–12)
- $1,500/month (Months 13–24)
-
$2,250/month (Months 25+)
OR - 7.5% of Gross Sales
Item 19 Disclosure: Yes